https://nova.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:14341 Wed 11 Apr 2018 16:06:08 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:30926 PR isoform expression ex situ and, further, to determine if conditions approaching the in vivo environment stabilise PR isoform expression in culture. Methods: Term nonlaboring human myometrial tissues were cultured under specific conditions: serum supplementation, steroids, stretch, cAMP, PMA, PGF_2α, NF-κB inhibitors, or TSA. Following 48 h culture, PR-T, PR-A, and PR-B mRNA levels were determined using qRT-PCR. PR-A/PR-B ratios were calculated. Results: PR-T and PR-A expression and the PR-A/PR-B ratio significantly increased in culture. Steroids prevented the culture-induced increase in PR-T and PR-A expression. Stretch blocked the effects of steroids on PR-T and PR-A expression. PMA further increased the PR-A/PR-B ratio, while TSA blocked culture-induced increases of PR-A expression and the PR-A/PR-B ratio. Conclusion: Human myometrial tissue in culture undergoes changes in PR gene expression consistent with transition toward a laboring phenotype. TSA maintained the nonlaboring PR isoform expression pattern. This suggests that preserving histone and/or nonhistone protein acetylation is critical for maintaining the progesterone dependent quiescent phenotype of human myometrium in culture.]]> Wed 11 Apr 2018 14:38:10 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:24546 In vitro sperm storage is a necessary part of many artificial insemination or in vitro fertilization regimes for many species, including the human and the horse. In many situations spermatozoa are chilled to temperatures between 4 and 10°C for the purpose of restricting the metabolic rate during storage, in turn, reducing the depletion of ATP and the production of detrimental by-products such as reactive oxygen species (ROS). Another result of lowering the temperature is that spermatozoa may be "cold shocked" due to lipid membrane phase separation, resulting in reduced fertility. To overcome this, a method of sperm storage must be developed that will preclude the need to chill spermatozoa. If a thermally induced restriction-of-metabolic-rate strategy is not employed, ATP production must be supported while ameliorating the deleterious effects of ROS. To achieve this end, an understanding of the nature of energy production by the spermatozoa of the species of interest is essential. Human spermatozoa depend predominantly on glycolytic ATP production, producing significantly less ROS than oxidative phosphorylation, with the more efficient pathway predominantly employed by stallion spermatozoa. This review provides an overview of the implications of sperm metabolism for in vitro sperm storage, with a focus on ambient temperature storage in the stallion.]]> Wed 11 Apr 2018 14:27:26 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:20808 Wed 11 Apr 2018 12:16:47 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:18878 Wed 11 Apr 2018 11:58:12 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:34942 Wed 09 Feb 2022 15:56:55 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:25973 Wed 04 Sep 2019 10:40:09 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:35024 Thu 21 Oct 2021 12:46:07 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:37077 Thu 13 Aug 2020 16:17:43 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:19422 Sat 24 Mar 2018 07:51:58 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:22691 Mon 11 Mar 2019 12:13:39 AEDT ]]>